Advanced Confocal Microscopy Workshop Brings International Expertise to Stellenbosch

Stellenbosch University, in partnership with the Institute of Physical Chemistry at Ludwig Maximilian University of Munich (LMU), recently hosted an intensive hands-on workshop focused on advanced confocal microscopy and state-of-the-art image correlation techniques.

The multi-day programme brought together leading international and local experts to deliver in-depth theoretical lectures and practical training for participants working in fluorescence microscopy, biophysics, and advanced imaging.

Lectures were presented by Prof Don Lamb and Ecenaz Bilgen from the Fluorescence Applications in Biology Lab (FABLab) at LMU, Dr Tawanda Sininga from the Department of Biochemistry at Stellenbosch University, and Lize Engelbrecht from the Stellenbosch University Central Analytical Facilities.

Prof Don Lamb and Ecenaz Bilgen from LMU presenting on FCS, RICS and FRET techniques to a group of participants visiting from various institutions, including UKZN and University of Venda.

Key theoretical sessions explored cutting-edge topics such as single-molecule Förster Resonance Energy Transfer (smFRET),

pulsed interleaved excitation (PIE), and fluorescence correlation spectroscopy combined with raster image correlation spectroscopy (FCS-RICS), among others.

The workshop also featured special guest talks highlighting the scientific applications of fluorescence and confocal microscopy across diverse disciplines. These were delivered by Prof Ben Loos from the Department of Physiology, Prof Rensu Theart from the Faculty of Engineering, and Prof Lyn-Marie Birkholtz from the Department of Biochemistry.

Alongside the lectures, attendees received structured hands-on experience through two practical training streams:

• Computer and software training, including data analysis workflows and computational simulations • Laboratory-based training using advanced fluorescence microscopy instrumentation

The workshop aims to strengthen international collaboration while equipping South African researchers and postgraduate students with high-level skills in quantitative fluorescence microscopy. Organisers note that the programme reflects a growing demand for specialised imaging capabilities in the life sciences, engineering, and physical chemistry.

The event underscores Stellenbosch University’s ongoing commitment to fostering research excellence through global partnerships and access to cutting-edge scientific methodologies.