The University of Pretoria houses electron and light/fluorescence microscopes in different locations.
The central Laboratory for Microscopy and Microanalysis at the University of Pretoria is operated as an independent laboratory and provides a range of services to students and staff members from all faculties. Users without university affiliation are also accommodated. All facilities are available during working hours to approved users.
The microscopy hub was established in 2016 within the Centre for Neuroendocrinology with the NRF-sponsored purchase of a Zeiss Laser Scanning Microscope LSM800. The LSM800 is equipped with atmospheric control (Temperature and CO2) and there is a POCmini 2 incubation chamber available for real-time imaging. With this equipment, we can track protein expression in live cells, and perform Foster resonance energy transfer (FRET) and Fluorescence recovery after photobleaching (FRAP) experiments. While the microscope will mainly be used by the Centre other groups and researchers within the faculty and within the university are welcome to make use of it. Training and supervision is available and advice and help is given with regards to the preliminary steps for successful imaging experiments.